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Posted 1y ago

Senior Bacteriology Technician

@ Goldbelt Apex
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Fort Detrick, Maryland, United States
$80k-$90k/yrOnsiteFull Time
Responsibilities:preparing reagents, plating plates, isolating plasmids
Requirements Summary:Bachelor’s in a related field; 7+ years related experience; SECRET clearance eligibility; PRP enrollment preferred.
Technical Tools Mentioned:ELISA, PCR, Luminex, Flow Cytometry, BD FACS Calibur, BD FACS Canto II, Aseptic technique
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Job Description

Please note that this position is contingent upon the successful award of a contract currently under bid.

 

Goldbelt Apex, a part of the Healthcare Technology Transformation Group, is a data-focused company dedicated to process and quality in every aspect. As experts in healthcare IT experts, Apex is committed to building systems for healthcare organizations to seamlessly communicate and exchange data across different systems and devices. 

 

Summary:

The Senior Bacteriology Technician shall assist the Lead/PI in Molecular Microbiological Experiments aimed at defining elements and factors involved in bacterial virulence.  Assist in approximately 2 animal protocols conducted by other investigators. 

Essential Job Functions:

  • Shall prepare approximately 10 different Reagents / Media for the lab (including R Medium, DMEM/FBS, LB Broth, Sodium Bicarbonate, APS LB Agar, BHI, etc). 
  • Contractor shall plate approximately 100 LB plates for use for Ames sporulation isolation and purification. 
  • Contractor shall isolate and purify approximately 10 plasmid DNA specimens. 
  • FITC label and count approximately 10 Ames samples to check ratio of Bacilli: Spores over multiple time points. 
  • Contractor shall order lab supplies for BSL-2 and BSL-3 laboratories including common supplies, custom orders, and equipment – approximately 400 items ordered along with 5 pieces of equipment. 
  • Present overview of data collected from Opsonic Adherence and ELISA at group meeting; present progress reports to supervising investigator.  Use computer to prepare graphs and write reports for supervising investigator. 
  • Contractor shall perform approximately 100 Immunological, antibody determinations. 
  • Contractor shall perform approximately 100 ELISAs on mouse, nonhuman primate, and/or human serum. 
  • Contractor shall perform approximately 50 Cytokine analyses. Measures cytokines in mouse, nonhuman primate, and/or human serum.  Measures cytokines in cell cultures and cell extracts.  Uses a Luminex instrument for analysis. 
  • Contractor shall perform approximately 50 Flow Cytometry tasks, determining cellular distribution in mouse samples and intracellular cytokine expression.  Uses a BD FACS Calibur and BD FACS Canto II to analyze cellular distribution. 
  • Perform other duties as necessary. 

Necessary Skills and Knowledge:

  • Expertise in laboratory safety and aseptic techniques. 
  • Proficiency in biological assay procedures (ELISA, PCR). 
  • Ability to manage sample tracking and recordkeeping. 
  • Possesses experience, skill and expertise with a large variety of the field's concepts, practices, and procedures.  

Minimum Qualifications:

  • Bachelor’s degree in a related field. 
  • Minimum 7 years of related experience. 
  • Ability to obtain and maintain a SECRET security clearance eligibility, with no access to classified information, is required. 
  • Enroll in the Personnel Reliability Program (PRP), if required. 

Preferred Qualifications:

  • ACTIVE SECRET is preferred 
  • Ph.D. preferred

Pay and Benefits

The salary range for this position is $80,000 - $90,000 annually. 

 

At Goldbelt, we value and reward our team's dedication and hard work. We provide a competitive base salary commensurate with your qualifications and experience. As an employee, you'll enjoy a comprehensive benefits package, including medical, dental, and vision insurance, a 401(k) plan with company matching, tax-deferred savings options, supplementary benefits, paid time off, and professional development opportunities.